[Humana Press Inc.]
Kategoria: Książki / Literatura obcojęzyczna
pitfalls. Comprehensive and cutting-edge, Germ Cell
Protocols offers both novice and established researchers a
gold-standard collection of hard-to-find methods of high impact
research, diverse...
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For volume 2 alone:§A gold-standard collection of readily
reproducible techniques for the molecular and genetic analysis of
germ cells in a variety of different reproductive systems. Volume
2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and
Cloning focuses on molecular egg analysis, live egg imaging,
nuclear cloning, oocyte cryopreservation, and nuclear transfer.
Highlights include the identification and characterization of
Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and
cloning in the field of trophoblast/placental development, and
selective ablation. Each readily reproducible protocol is described
in step-by-step detail and contains an introduction outlining the
principle behind the technique, lists of equipment and reagents,
and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Germ Cell Protocols offers both
novice and established researchers a gold-standard collection of
hard-to-find methods of high impact research, diverse procedures
that are easy-to-follow, well-illustrated, and allow a
cross-species transfer of knowledge from lower vertebrates to
higher mammalian systems.§For both volumes:§A gold-standard
collection of readily reproducible techniques for the molecular and
genetic analysis of germ cells in a variety of different
reproductive systems. The methods cover sperm and egg activation,
motility, fertilization, nuclear development, nuclear cloning, the
molecular characterization of specific events, and the imaging of
cell structures. Volume 1: Sperm and Oocyte Analysis focuses on
sperm cells, oocyte analysis, oocyte maturation, fertilization, and
preparation techniques. Volume 2: Molecular Embryo Analysis, Live
Imaging, Transgenesis, and Cloning contains methods for molecular
egg analysis, live egg imaging, nuclear cloning, oocyte
preservation, and nuclear transfer. §Comprehensive and
cutting-edge, Germ Cell Protocols offers both novice and
established researchers a gold-standard collection of hard-to-find
methods of high impact research, diverse procedures that are
easy-to-follow, well-illustrated, and allow a cross-species
transfer of knowledge from lower vertebrates to higher mammalian
systems.